| Authors |
Anatoliy S. Barkhatov, Postgraduate student, junior researcher, Research Center of Genomic Selection, Belgorod State National Research University (85 Pobeda street, Belgorod, Russia), E-mail: barkhatov@bsu.edu.ru
Eduard A. Snegin, Doctor of biological science, professor, the director of the Research Center of Genomic Selection, Belgorod State National Research University (85 Pobeda street, Belgorod, Russia), E-mail: snegin@bsu.edu.ru
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| Abstract |
Background. Morphological features are one of the most important markers for bioindication. Among them, the polymorphism of phenotypic traits occupies a special place. In tailless amphibians, dorsal colouration variability is widespread: the presence or absence of a light dorsomedial stripe or dark spots. The phenotypic composition is known to change in the impacted areas. The purpose of the study was to analyze the phenotypic structure of Pelophylax esculentus complex populations in the urbanized landscapes in the South Central Russian Upland. This study is a part of the comprehensive analysis of the population structure of green frogs in the South Central Russian Upland. Materials and methods. A total of 748 individuals were sampled from 35 localities in the South Central Russian Upland. Collection points differed in the degree of anthropogenic pressure. Dorsal coloration variations were analyzed according to the generally accepted grading. Analysis of phenotypes diversity was performed according to the indicators proposed by L.A. Zhivotovsky. Results. Four main phenotypes “Maculata” (M), “Striata” (S), “Punctata” (P), “Burnsi” (B), and their combinations SM, SP and SMP were registered in the study area. The highest phenotypic diversity was observed in natural or sparsely disturbed biotopes “Sazon” (6.815 ± 0.251), “Aidar” (6.527 ± 0.306), “Goluboi Dunai” (6.43 ± 0.418), while under anthropogenic pressure an increase in the proportion of rare phenotypes was observed. The principal component analysis has demonstrated the phenotypic similarity of most of the populations. At the same time, unique groups were found in both impact and natural habitats, which may indicate an effective compensatory response of the green frog populations in the study area to anthropogenic pressures. Conclusions. The results obtained confirm the data of the previous studies on the phenotypic polymorphism of green frogs, according to which the phenotypic diversity declines and the proportion of rare phenotypes increases under conditions of anthropogenic pressure. In addition, in a number of populations, both in urbanized and relatively clean biotopes, unique combinations of phenotypic frequencies are observed, indicating active adaptation of green frogs to changing environmental conditions.
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| References |
1. Lada G.A. Tailless amphibians (Anura) of the Russian Plain: variability, speciation, habitats, problems of protection. DSc abstract. Kazan, 2012:48. (In Russ.)
2. Zhelev Z.M., Arnaudova D.N., Popgeorgiev G.S., Tsonev S.V. In situ assessment of health status and heavy metal bioaccumulation of adult Pelophylax ridibundus (Anura: Ranidae) individuals inhabiting polluted area in southern Bulgaria. Ecological Indicators. 2020;115:1–15. doi:10.1016/j.ecolind.2020.106413
3. Ryzhov M.K. Amphibians and reptiles of the Republic of Mordovia: spreading, allocation, trophic relations and the state of protection. PhD abstract. Tolyatti, 2007:19. (In Russ.)
4. Spirina E.V. Amphibians as a bioindication test system for ecological assessment of aquatic habitats. PhD abstract. Ulyanovsk, 2007:22. (In Russ.)
5. Kuzovenko A.E. Ecological and faunistic characteristics of amphibians in urbanized territories of Samara region. PhD abstract. Tolyatti, 2018:19. (In Russ.)
6. Peskova T.Yu. Struktura populyatsiy zemnovodnykh kak bioindikator antropogennogo zagryazneniya sredy = The structure of amphibian populations as a bioindicator of anthropogenic pollution of the environment. Moscow: Nauka, 2002:132. (In Russ.)
7. Vershinin V.L. Ecological features of amphibian populations in urbanized areas. DSc abstract. Ekaterinburg, 1997:47. (In Russ.)
8. Zaripova F.F., Yumagulova G.R., Fayzulin A.I. Characteristics of the state of Rana ridibunda Pallas, 1771 (Anura, Amphibia)population in the Republic of Bashkortostan on the polymorphism of the back color pattern. Izvestiya nauchnogo tsentra Rossiyskoy akademii nauk = Proceedings of scientific center of the Russian Academy of Sciences. 2009;11(1):78–82. (In Russ.)
9. Vershinin V.L. Morfa Striata and its role in the pathways of adaptationogenesis of the genus Rana in the modern biosphere. Doklady akademii nauk = Reports of the Academy of Sciences. 2004;396(2):280–282. (In Russ.)
10. Zamaletdinov R.I. Phenotypic structure of populations of green frogs in urbanized areas. Povolzhskiy ekologicheskiy zhurnal = Volfa ecological journal. 2002;(2):163–165. (In Russ.)
11. Nikashin I.A. Ecological and morphological characteristics of Rana ridibunda Pall. population as a means of assessing anthropogenic impact on aquatic ecosystems (by the example of Lipetsk region). PhD abstract. Lipetsk, 2007:17. (In Russ.)
12. Berger L. Morphology of the F1 generation of various crosses within Rana esculenta – complex. Acta Zoologica Cracoviensia. 1968;13:301–324.
13. Tunner H.G. Die klonale Struktur einer Wasserfroschpopulation. Zeitschrift für zoologische Systematik und Evolutionsforschung. 1974;(12):309–314. doi:10.1111/j.1439-0469.1974.tb00173.x
14. Akin C., Bilgin C.C., Beerli P. [et al.]. Phylogeographic patterns of genetic diversity in eastern Mediterranean water frogs were determined by geological processes and climate change in the Late Cenozoic. Journal of Biogeography. 2010;37(11):2111–2124. doi:10.1111/j.1365-2699.2010.02368.x
15. Plötner J. Die westpaläarktischen Wasserfrösche: von Märtyrern der Wissenschaft zur biologischen Sensation. Bielefeld: Laurenti, 2005:160.
16. Lada G.A. Central European green frogs (hybridogenic complex Rana esculenta): introduction to issues. Flora i fauna Chernozem'ya = Flora and fauna of the Chernozem region. Tambov, 1995:88–109. (In Russ.)
17. Ivanov A.Yu. Molecular genetic and ecological features of the distribution of cryptic forms of the marsh frog in the eastern part of the range. PhD dissertation. Penza, 2019: 137. (In Russ.)
18. Ivanov A.Yu., Ruchin A.B., Fayzulin A.I. [et al.]. The first record of natural transfer of mitochondrial DNA from Pelophylax cf. bedriagae into P. lessonae (Amphibia, Anura). Nature Conservation Research. Zapovednaya nauka = Nature Conservation Research. Conservation science. 2019;4(2):125–128. doi:10.24189/ncr.2019.020
19. Zamaletdinov R.I. Ecology of amphibians in a big city (by the example of Kazan). PhD abstract. Kazan, 2003:24. (In Russ.)
20. Borkin L.Ya., Tikhenko N.D. Some aspects of morphological variability, color polymorphism, growth, population structure and daily activity of Rana esculenta at the northern border of the range. Ekologiya i sistematika amfibiy i reptiliy = Ecology and taxonomy of amphibians and reptiles. 1979;89:18–54. (In Russ.)
21. Lada G.A., Sokolov A.S. Metody issledovaniya zemnovodnykh: metod. posobie = Research methods of amphibians: methodological allowance. Tambov, 1999:75. (In Russ.)
22. Zhivotovskiy L.A. Indicator of similarity of populations by polymorphic traits. Zhurnal obshchey biologii = Journal of General Biology. 1979;40(4):587–602. (In Russ.)
23. Zhivotovskiy L.A. Populyatsionnaya biometriya = Population biometrics. Moscow: Nauka, 1991:276. (In Russ.)
24. Barkhatov A.S., Snegin E.A., Yusupov S.R. Genetic structure of populations of the green frog complex (Pelophylax esculentus complex) in the South Central Russian Upland. Ekologicheskaya genetika = Ecological genetics. 2021;19(2). (In Russ.). doi:10.17816/ecogen48555
25. Vershinin V.L. Biota urbanizirovannykh territoriy = Urban biota. Ekaterinburg, 2007:73. (In Russ.)
26. Fayzulin A.I., Kuzovenko A.E. The use of amphibians in monitoring the state of the environment in the Samara region: phenetic structure of populations. Izvestiya Samarskogo nauchnogo tsentra Rossiyskoy akademii nauk = Proceedings of Samara scientific center of the Russian Academy of Sciences. 2012;14(1):829–833. (In Russ.)
27. Fayzulin A.I., Zaripova F.F., Khusainova I.M. Peculiarities of striatal polymorphism in marsh frog populations of Rana ridibunda Pallas, 1771 (Anura, Amphibia) in the Republic of Bashkortostan. Izvestiya Samarskogo nauchnogo tsentra Rossiyskoy akademii nauk = Proceedings of Samara scientific center of the Russian Academy of Sciences. 2013;15(3):452–458. (In Russ.)
28. Kuzovenko A.E. Ecological and faunistic characteristics of amphibians in urbanized territories of the Samara region. PhD dissertation. Tolyatti, 2018:211. (In Russ.)
29. Lebedinskiy A.A. Adaptation of amphibians to the conditions of an urbanized territory. Problemy regional'noy ekologii zhivotnykh: tez. dokl. Vseros. konf. zoologov pedinstitutov = Issues of regional animal ecology: proceedings of the All-Russian conference of zoologists of pedagogical institutes. Vitebsk, 1984:106. (In Russ.)
30. Lebedinskiy A.A., Pomorina E.N. Some features of the grass frog population in connection with its habitat in an urbanized area. Vestnik Nizhegorodskogo universiteta imeni N.I. Lobachevskogo = Bulletin of Lobachevsky University. 2008;(2):91–95. (In Russ.)
31. Lada G.A. On the genetic polymorphism of the marsh frog (Rana ridibunda) in the Central Black Earth Region. Fenetika prirodnykh populyatsiy: materialy IV Vseros. Soveshch. = The phenetics of natural populations: proceedings of the 4th All-Russian conf. Moscow, 1990:151–152. (In Russ.)
32. Shabanov D.A., Litvinchuk S.N. Green frogs: life without rules or a special way of evolution? Priroda = Nature. 2010;(3):29–36. (In Russ.)
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